Protein Assays

Protein Assays Below is a list of assays for the determination of protein concentration in a solution. This list includes the sensitivity range, volume/ touchstone of try enquireed, subjective comments on accuracy and convenience, and major interfering agents. adjective details, equipment requirements, and references are outlined in the individual assay documents. The criteria for substitute of a protein assay are usually based on convenience, availability of protein for assay, presence or absence of interfering agents, and lack for accuracy. For example, the Lowry method is very sensitive just is a twain step procedure that requires a minimum of 40 minutes incubation time. The Bradford assay is more sensitive and can be read within 5 minutes, however proteins with low arginine get wind will be devalued. globally, estimates are more accurate for gnarly mixtures of proteins. Estimates of concentration of pure proteins can be very loose depending on the principl e of the assay, unless the same pure protein is used as a standard. Criteria will be discussed in the individual documents.

Because severalise proteins have different amino acid compositions, the sensitivity of colorimetric assays to individual proteins may vary widely. The most reprodicible results are obtained with standards put forward up of a mixture of proteins that is as similar as realistic to the unknown. For most purposes, a relative amount is near enough, but the standard used should be reported. For example, the Bradford assay is more more sensitive to bovine serum albumin (BSA) than to immunogl obulin G (IgG), so that with IgG the investi! gator is likely to overreckoning the amount of protein in a sample. With BSA the investigator is likely to underestimate the amount. General Reference: Stoscheck, CM. Quantitation of Protein. Methods in Enzymology 182: 50-69 (1990).If you want to get a broad essay, order it on our website: OrderCustomPaper.com

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